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  • Nemesio Villa-Ruano

    Mon Nov 13 2023

    Nutraceutical Activity of Anthocyanins from the Edible Berries of Rhamnus pompana


    We present the inhibitory properties of the R. pompana anthocyanin fraction (RPAF) and its major constituents on alpha-glucosidase (AG), pancreatic lipase (PL), HMG-CoA reductase, and ornithine decarboxylase (ODC). The effect of RPAF was also evaluated in ICR male mice subjected to oral glucose tolerance test (OGTT) and hypercaloric/atherogenic diet for 30 days. RP-HPLC/MS profiling revealed that RPAF contained five major anthocyanins and induced slight inhibition on PL and HMG-CoA reductase (IC50, 245–338 μg mL−1) whereas strong activity on AG and ODC (IC50, 130–133 μg mL−1) was observed. Kinetic studies and molecular docking with pelargonidin-3-O-rutinoside (P3R) on ODC, revealed changes in Km (0.9514–0.9746 mM) and Vmax (1.96–2.32 μmol mg−1 min−1) suggesting mixed inhibition and molecular interaction with two active sites of ODC. P3R showed antiproliferative activity (IC50, 46.5 μM) and decreased polyamine accumulation in DLD-1 cells.


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