In this European Commision funded Marie Skłodowska-Curie Individual Fellowship project I have been working on the exciting developments in the field of organic room temperature phosphorescence (RTP) nanocrystals and their potential applications in bioimaging. Organic RTP materials have gained significant attention due to their high brightness and ultra-long lifetime features, making them useful in many advanced applications, including optoelectronics, bioimaging, and photomedicine. However, processing of nanoform organic RTPs and scalable synthesis lag behind other forms of RTPs, leaving the chances for many applications nearly uncharted. The EC-funded FUNCrystals project aims to develop functional organic RTP nanocrystals (NCs) for two-photon phosphorescence lifetime imaging microscopy (2P-PLIM) based bioimaging applications. My project proposes three main activities are ORTP-NCs synthesis, biological studies and 2P-PLIM usage. These activities include developing a synthesis process.